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Thank you for this very detailed explanation of what is happening. This does explain quite nicely what I have been thinking and trying to understand. It does explain why once fish and the food they eat is added it often causes at least a mini cycle in a tank that was showing 0 ammonia, 0 nitrites and some nitrates after a long drawn out cycling process. Or if a cycle seems stalled, adding fish food gets it going in the right direction.I have been keeping fish for the past 60 years including both freshwater and saltwater aquaria. I have never cycled a tank with ammonia and I would never consider doing so. Yes, feeding ammonia to the tank will allow the growth of nitrifying bacteria that will convert ammonia to nitrite and then allow growth of the bacteria that convert nitrite to nitrate. However, you are starting in the middle of the cycle, not at the beginning. In an aquarium, the nitrogen cycle does not begin with ammonia. It begins with proteins that are formed from amino acids. These amino acids are organic compounds that contain both hydroxyl (COOH) and amine (NH2) groups. It is these amine groups that are the source of nitrogen in the aquarium. When protein is consumed by our fish, the amino acids are metabolized and converted to ammonium ion in the liver of the fish. The ammonium ion is converted to urea in both the liver and kidneys of the fish. The urea is excreted as part of the waste products.
Urea is converted to ammonia by an enzyme contained in bacteria and plants. Thus, our aquaria needs a healthy colony of the bacteria responsible for breaking down the urea. When you start the nitrogen cycle with ammonia, you are limiting the growth of these very important bacteria. Live plants also consume urea. That is why many fertilizers contain urea as part of the available nitrogen.
Once the urea has been converted to ammonia, then the nitrifying bacteria take over and convert the ammonia to nitrite and then to nitrates.
Remember, though that when you talk about the nitrogen cycle, what goes around has to come around. The next step in the nitrogen cycle is the conversion of nitrates to nitrogen gas by anaerobic bacteria called denitrifying bacteria. Some biological media are specifically designed to foster the growth of anerobic bacteria deep within the structure of the media. In a saltwater aquarium, a deep sand bed or live rock provide such structures. In a freshwater aquarium, filter media like Seachem De-nitrate and Matrix are designed to do this. In nature this process also occurs in swamps and stagnant ponds.
The cycle continues in nature with legumes that absorb nitrogen from the air and with the help of nitrogen-fixing bacteria in root nodules are able to convert atmospheric nitrogen into amino acids/proteins thus bringing us back to the very beginning of the cycle.
Thus, in order for a tank to be considered fully cycled, you need to start with either protein or urea, not ammonia. Some people start with a small piece of frozen fish or shrimp to provide protein. Some people add urine to the tank to provide urea (yes, it will work). I take the less messy route and start by adding a pinch of high protein fish food to the tank each day. Because the food is broken down slowly by bacteria, you do not need to worry about dosing levels unless you dump way too much food into the tank.
I do realize what most of us have isn't a full cycle since most of us never manage to grow the bacteria that removes the nitrates. I never get into that part of it since I've not accomplished it myself so it is above my knowledge level.
Like you I have never cycled a tank with ammonia but since so many folks do I have tried to learn all I can about doing so. I just felt something was missing in the process.
