Hey so general info I found online from years ago
"Add dechlorinator/dechloraminator of choice . Water must be conditioned before using Bio-Spira™ or the bacteria will die from the chlorine or chloramines normally present in tap water. However, ammonia-removing products like Ammo-Lock and Bio-Safe, which will give the bacteria of Bio-Spira nothing to feed on, must be avoided. However, water conditioners such as AmQuel®, Prime™ and Ammonia Detox may still used and should be used if you have to neutralize chloramines in your tap water"
In any case, if you don't treat the tap water then the chlorine/chloramine will kill the nitrifying bacteria.