CindiL
- Thread Starter
- #41
Makes me wonder how decent Seachem Stability product is with beneficial bacteria.
Q: How does Prime make a difference in reducing Nitrates?
A: The detoxification of nitrite and nitrate by Prime (when used at elevated levels) is not well understood from a mechanistic standpoint. The most likely explanation is that the nitrite and nitrate is removed in a manner similar to the way ammonia is removed; i.e. it is bound and held in a inert state until such time that bacteria in the biological filter are able to take a hold of it, break it apart and use it. Two other possible scenarios are reduction to nitrogen (N2) gas or conversion into a benign organic nitrogen compound.
I wish we had some more "concrete" explanation, but the end result is the same, it does actually detoxify nitrite and nitrate. This was unexpected chemically and thus initially we were not even aware of this, however we received numerous reports from customers stating that when they overdosed with Prime they were able to reduce or eliminate the high death rates they experienced when their nitrite and nitrate levels were high. We have received enough reports to date to ensure that this is no fluke and is in fact a verifiable function of the product.
All very valid questions posed. In reference to "overdose" part. If I'm not mistaken the instructions on the bottle of Prime state to use 5x the recommended dosage for the tank treated to protect against nitrites. Anecdotally I've read on the forum that this dosage is only effective for nitrite levels at 1.0 or lower for 24 hours
I have asked Seachem this before. The regular dose stated on the label will take care of 1.0 ammonia + nitrites. Anything higher you have to use more than the regular dosage.
Based on that though, anyone cycling without TSS or with Stability or nothing, no matter what should be doing large water changes when ammonia + nitrite gets up 1.0. I only have anecdotal from the goldfish forum and I've seen literally 100's of people cycle with prime alone with no fish harm (that was visible) or death. It would be nice if there was actually some scientific evidence of this though!
At this point, I would leave the tank alone. It is perfectly normal for the ammonia and nitrite levels to rise to around 1.5 ppm, maximum, and gradually lower over a period of a week or two. Remember, this is not a chemical, but a bacteria that you have added to the tank. It takes time for it to do its job. Also, low levels of ammonia like you have described are not harmful to the fish. I suspect the oto died because they ONLY eat algae, and will starve to death in new aquaria. BREX
January 28, 2015
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If you did water changes when using TSS when levels reached 1ppm, you'd have to wait 24-48 hours and redose the TSS and potentially still harm the food source for any TSS that was remaining when you dose Prime. You would negate the purpose of using TSS to cycle the tank because the tank isn't likely to cycle in the 2 week time frame, because you would be regularly removing much of the bacteria with each water change, and then waiting a couple of days to bring bacteria levels up again.
The real fact of the matter is, if a person doesn't want their fish to have to go through an aquarium cycle, and avoid subjecting them to ammonia and nitrites, then a fishless cycle is the best way to go. IMO, other than with the exception of having a good source of seeded media in which you can obtain an instant cycle on another tank with, fishless cycling is the best choice period.
I assume people on the goldfish forum are cycling tanks with more sensitive fish present and not only goldfish?
I actually said without TSS, ha, ha, bet your eyes skipped right over that I probably should have worded it differently.
But yes totally agree on fishless cycling as fish-in cycling is stressful.
No, just goldfish as its never recommended to give them other tank mates. Most people buy the goldfish before they learn about the nitrogen cycle.
I totally would have. I have a surplus of guppy fry and way too much prime. But I just set up my 5.5 gallon with TSS.
On the other hand, I suppose it wouldn't be too hard to tear it down and rinse off the bio balls.
If you are all really interested in the results, I can totally conduct science and report daily @.@
You're such a trooper oOBlueOo, but dang, I have to say that I'm trying not to torture too many more fish in my lifetime. I'd have to have more certainty that Prime is able to keep them protected than I do. Like I said, when I encounter people with ammonia and nitrite issues, the first thing I try to have them do is get the levels down to .25 or below, even if they use Prime to help detoxify the rest for 24 hours, and I try to get them to keep it that low with water changes for the duration of the cycle. What we are proposing are higher levels than that. It would be nice to be able to get the information without putting fish at risk solely for the purpose of conducting this test.
I guess my vote is, that I would prefer you didn't, but I would also like to see how much longer than average a cycle takes (if any) when cycling with Prime. I know cmclien said it took her about 12 weeks, and I wonder if that is common.
There must be other ways of doing this?
So would stability be used at this time, also? Or just strictly prime?
Well, cmclien will give you the guideline. I don't think she has 75% water changes in mind, because she has higher levels in mind than what she had when she cycled with Prime before.
Do you have a KH test kit? It would be good to know what level the KH is at. If it's too low, there is more chance of the pH dropping too far and interfering with the cycle. What is the level of nitrates in the RO blend, and what water are the fry in now?
OK, so you'd be using the same water mixture they are currently use to, with a GH 9, KH 8 and pH 7.4. API test kits for GH/KH as well as ammonia etc.? What is the temp of the tank going to be?
I'd like to use the water they are use to, because we're not out to try to kill your fish. It does mean the the ammonia won't be as toxic as in higher pH levels, but if these numbers look good to cmclien, then we will go with them. If she would like to see the pH higher for toxicity purposes, then maybe we could get you to slowly add a little more of your hard water to the tank, in a way that you would still know the ratio of hard water to RO for water changes. The nitrates would be slightly higher, but what ever level they are at before the cycle is started, would be ground 0, so we'd still know when you start to show nitrates from the cycle.
Are you sure you want to do this oOBlueOo? It could take a few weeks to cycle it this way..... it's quite a commitment on your part. Mind you... if you decided you had enough, I don't think either one of us would try to stop you from throwing in a bottle of TSS.
Yes I'm using liquid API tests for everything. The temperature I plan on keeping the same, 80*F.
I have work this morning, but I'll get some more RO water after and wait for cmclien to answer before tearing down and setting up again.
And yes I can do this. Honestly. I love lab work
Hi, sorry for the delay. So here is the formula recommended to everyone which they came up with after my tank was already cycled.
If ammonia + nitrites is less than 1.0 - double dose Prime and re-check in 24 hours
If ammonia + nitrites is 1.0 or greater - do a very large water change, like 80-90% matching ph and temp.
Double dose prime and re-check in 24 hours.
and so on until cycled. Now I do think the double dose was partly because of the huge bio-load of goldfish and the possibility of spikes in both ammonia and nitrites so I am not really sure you need to do that? But maybe with enough guppies in a 5 gallon it will be a similar bio-load comparison. Seems like with goldfish and using that formula most people were doing the large water changes every 2 or 3 days and/or daily once the nitrites spiked because they get so high so fast.
My ph is 8.3 which may have delayed my cycle also? not sure on that one. I would say if your natural ph is higher I'd skip the R/O and just go from a base of 5 nitrates. Up to you guys though as you know better.
Also because it was goldfish and I was cycling in the summer my temperature was about 74-75 without a heater. So I would say go down to 76 if that's alright for the guppies, which I think they have a wide range of acceptable temperatures right?
This will be really great to finally know the truth about Prime and if nitrite and ammonia are truly detoxified.
Wow, at my ph and temp less than .25 ammonia is toxic to my fish! Good thing I have a cycled tank!
I wouldn't add more fish because it would be another factor for possible death if the store fish contaminate your current fish with something.
Temperature also plays a part in the toxicity of ammonia, so if you'll be keeping the 7.4pH I wouldn't change the temp, to help keep the toxicity a little higher. I'll include here a toxicity chart so that everyone can see the association between ammonia level, ph, temperature and toxicity. You'll have to scoll down a ways but it's there.
Okay. So just I clarify, we're going with a ph of 7.4, a water ratio of 3 g RO/ 1.5 g tap, and a temp of 80*f to keep ammonia less toxic?
I went back and I looked at the chart closer. So the lower numbers with the higher temp mean it takes less ammonia to be toxic. Or that 1.0ppm ammonia is more toxic in 80* and less toxic 70*....
Is that what it means? If it does, I think I understand now.
Okay so a lower ph and lower temp?
Sorry for all the questions. I just want to make sure I get this right.
I think she means (??) because your ph is at 7.4 than a higher temp would be more comparable to a higher ph and a lower temp if that makes sense. If your nitrates are only 5 out of the tap (is that what you said?) I'd still drop the R/O to up the ph some. What is your ph without the R/O?
Nope. My ph in the tank with the RO water is 5. Out of the tap, it's illegal. Well, it would be if it was city water.
Nope. My ph in the tank with the RO water is 5. Out of the tap, it's illegal. Well, it would be if it was city water.