It's been 10 days since my addition of TSS and I don't think that it worked successfully in my tank. :'( I think it was because I changed the activated carbon in one of my filters and the all power to my tank was off for 5 hours, killing the beneficial bacteria. Here are the latest readings: Ammonia- 1.0 ppm Nitrite- 0 ppm? (slightly darker than the light blue on 0) Nitrate- between 20-40 ppm I know that now I have to do daily water changes with Prime. Can someone recommend a plan to me, like how much % to do each day, etc.? If it helps, two days ago I had an ammonia spike of 2.0-4.0 ppm and now it's gotten lower to 1.0 ppm. I've only seen nitrite twice (both times less than 1 ppm) and when nitrite would get to 0, there was an increase in nitrate. Also, if I turn off the power to my filters for about 20 minutes (that's how long a 50% w/c usually takes me) will the beneficial bacteria in the filter die? And when I add clean water to the tank during a water change, is it best that I add water conditioner when the tank is full or do I add water conditioner only to the new water and add that to the tank? Thanks.