Having some issues with my fishless cycle, and hoping for some insight! 40g breeder tank Aqueon Quiet Flow 75 filter Air bubbler running Temp 82-84F API master test kit Tank is about 50/50 fake/live plants - added two moss balls since picture was taken. Set tank up on 7/11, used aqueon water conditioner. Was using this as a guideline for fishless cycle: Ammonia instructions for a fishless cycle Tap water: 7.2ph, 0.25ppm ammonia, 2KH (was pale yellow at 1st drop, bright bright yellow at 3), 2GH. Water district does add chloramines, this was the most recent water report I could find: 7/14 Dosed ammonia to 4ppm. Don't have the brand handy, but it is free of surfectants, no bubbles, brand new bottle. Dosing with a syringe. Added 5ml every half hour and tested. Appears to be 10ml=1ppm for us.7/16 Added TSS+ (~100ml), however bottle had been sitting in mailbox and was pretty warm. Assuming most/all of bacteria died in transit. (Dumped in other half of bottle 7/17, ~150ml, just in case) Nitrates were reading 5ppm already at this point, though I don't recall if these tests were before or after I switched from strips to the API kit...7/22 ammonia was finally down to 2ppm.7/23, ammonia 1ppm. Still no nitrites, 5ppm nitrates. Dosed back to 4ppm ammonia.7/24, ammonia 2ppm. Dosed with TSS, having assumed prior bottle was DOA. (And because I'm impatient!?)7/26, ammo 2ppm, 0 nitrites, 5 nitrates7/28 Added more water conditioner after reading some posts about high chloramines possibly being the cause of not cycling/stalled cycling.7/31, ammo still sitting at 2ppm, 0 nitrites, 5 nitrates. After reading suggestions to bang the second bottle, retested nitrates (banged it quite a bit, then shook for 2 minutes before adding to tube), slightly darker than before, but still doesn't seem quite as dark as 10ppm on the chart. *sigh* Even if the TSS was a dud both times, shouldn't I still be seeing some bacteria growth and resultant parameter changes? And why would I have seen those ammonia numbers start to drop without any increase in nitrates?? I can't imagine I'm planted heavily enough to be consuming THAT many nitrates. Any suggestions on my next step? I was really hoping the TSS would have jump started things, as we were hoping to have at least a couple fish in the tank for my son's birthday next weekend. After hearing all the posts about people cycling with TSS so quickly, it's a bit frustrating to not be seeing any movement!