Sometimes when I test my water paramaters I don't dump the vials right away and their color will either not change or may darken slightly as they sit. Today however my nitrite and ammonia each tested 0.25 so I went about doing a pwc leaving the vials. I forgot about them and left them sitting a couple hours then glanced at them and my ammonia and nitrite had gone to zero while nitrate(10) remained unchanged. Is this proof that overdosing Prime really does detox ammonia and nitrite? I have been doing two 50% pwc a day and 5dpg Prime because nitrite was consistently going to 1 every 10hrs or so. I would always test before right after a water change then 10hrs later so Prime had never been in the water for 24hrs before I would freak out and do another water change because of the high nitrite. Last night however I ran out of stress zyme and decided to be strict and not test or change the water for 24hrs. I figured since I had only done one pwc in the morning I should add extra Prime for the half of the water I did not remove. I added 25 drops directly to the filter output to protect my fish just in case and they seemed to perk up instantly. This morning they were swimming around happy as can be and begging to be fed which I did as I was collecting water to test. This is the first time the test tubes have gone to zero after being left so I was wondering if anyone could tell me what, if anything it means? I usually won't note any changes to the regents after 5 minutes but this was different.