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Hello. I am new to this forum. I have had bad luck with Glofish. Before I can talk about our Glofish I will give a short history: We bought a New 10 Gallon Glofish tank kit at Petco (nearest store to us) and set it up on 6/10/19. The tank was bought and set up because we won 2 Goldfish at a fair that day. I know, I know this is not ideal but we did our best to have the fish survive as we were totally unprepared. 1 fish died before we could put it in the tank. We put the other Goldfish in the tank AFTER set up but it died overnight. (The tank was set up with Aqueon water conditioner and we followed the instructions on the bottle). Having spent a bit of money on the tank, conditioner, gravel etc., we decided to give it another go. By this time (6/11/19) I had started reading several web sites, including this one, and read about the nitrogen cycle. I decided to buy Tetra Safe Start (regular bottle, not the plus) and I added it to the tank as directed on the bottle. We did not add fish right away as I wanted to test the water and give the tank time to cycle. On 6/13/19 I tested the water by using Tetra test strips and levels were: Nitrate 20-40, Nitrite .5, Chlorine 0.5, KH 40, PH 7.8-8.4 and GH 150-300. Water appeared cloudy and I did not yet test for ammonia. Our kit came with a heater but I did not use it. It also came with a stick on strip thermometer and the temp was reading between 74-76. I continued to let tank cycle without fish. The water was not tested again until 6/16/19 (again all testing performed with Tetra test strips) and levels were as follows: Nitrate 20-40, Nitrite 0, Chlorine 0, KH 40-80, PH 7.2, GH 150-300, Ammonia 0. Water appeared very clear. Water was next tested on 6/21/19 and all levels were the same except for KH which was at 80. On 6/22/19, since levels appeared stable we decided to buy 5 Tetra Glofish from Petco (2 Longfin tetras, 3 reg tetras). I tested the water before we bought them and all levels were the same, this time with KH being back at 40-80. I asked for advice from the employee at Petco as to how to introduce our new fish to our tank. All I was told was to float the bag for 15 mins to get them used to the water temp. (Heater still not being used but temp in our tank was 74-76). I actually floated the bag for 45 mins and then around 5pm dumped out most of the water from their bag into our dirty water bucket but left a tiny bit of water from their bag and then released them into our tank so they could swim in since I didn't want to hurt them by using a net. (I have since read that was a very stupid thing to do by letting other tank water go into my tank!!!) Regardless, that was the method. By 9pm, 4 had died. With one blue tetra alive all i could think to do was add some water conditioner in case there was a spike in the water levels by adding 5 fish at once so I added 5ml of Aqueon water conditioner and also 10ml of API Quickstart to try to save the little guy. He lasted into the overnight some time, I last saw him alive at 11pm. By the morning of 6/23 he had died. On 6/23 I removed all fish from the tank. Water appeared cloudy and levels tested at: Nitrate 20-40, Nitrite 0, GH 150, Chlorine 0, KH 80-120, PH 7.2-7.8, Ammonia 0. On 6/23 I took fish back to Petco and got my money back. I also brought two water samples for them to test, one from when the 4 fish died on the night of 6/22 (and before I added water conditioner and API Quickstart) and one from the morning of 6/23 after having added conditioner and quick start. Both tested the same as the levels I took that morning - they also use Tetra test strips at their store. I decided to not buy more fish but to keep an eye on testing levels over the next week or so before proceeding as we don't want to kill any more fish! Also, I decided to put the heater in the tank on 6/23 since I and the Petco folks thought maybe it was the temp of the water that might've shocked them (theirs was more like 76-78 as i noticed that day and I think ours had been around 72-74). I also bought a regular thermometer that sits in the water (actually sits in tank but attached to glass with a magnet). On 6/24 and 6/25 all levels were Nitrate 20, Nitrite 0, GH 150, Chlorine 0, KH 40, PH 7.2, Ammonia 0. Water was clear and temp now stable at 78. I took a sample of my water to Petco since they said their Aquarium Specialist would be in that day and to ask him any questions I had. He tested my water and levels were the same. I also asked him to test their water, which he did, and levels were exactly the same. I did not buy fish but wanted to give tank more time to cycle. From 6/26 to 6/30 all levels were exactly the same. So on 6/30 we thought we would be ok to buy more Glofish. We went ahead and got 5 again (2 Longfin tetras, 3 Reg tetras). I also brought a sample of my water for them to test first which tested exactly the same as all my previous levels and I asked them to test their water before I brought the fish home (their testing levels were Nitrate 20, Nitrite 0, GH 300, Chlorine 0, KH 80, PH 6.8-7.2, Ammonia 0. I had previously bought the Doradon Acclimation System since after all my research and reading several websites seem to point to the fact that the first few Glofish probably died from not being properly acclimated. Note: I've also been keeping a notebook on my levels and taking notes on water clarity. We came home with our fish thinking FOR SURE they would now survive as we seemed to have done our homework on how to properly acclimate the fish and the our tank ready for them. At 5pm we followed the instructions for the Doradon system and acclimated them until 9pm just to be on the same side. (I dumped water from the bag periodically into our dirty water bucket) to help with this process. I also tested the water in the bag and the water in our tank and all levels were exactly the same- Nitrite 20-40, Nitrite 0, GH 150, Chlorine 0, KH 40-80, PH 7.2, Ammonia 0. Again, at 9pm we added the fish. They seemed fine at first although within an hour one was showing signs of stress as he was sitting at the bottom of the tank from time to time. This one fish seemed the most stressed anyway once he was put in the bag at Petco, so honestly I thought perhaps he wouldn't make it anyway. The other 4 were swimming calmly and exploring the tank. By 11 pm they all looked fine, they were not up at the top of the water gasping for air but looked calmed as they were swimming nicely in the tank. Note, we also had lights off during the acclimation and introduction to our tank so as not to stress them. By this morning 4 fish were dead, with one blue reg tetra left. I honestly do not know what happened! I thought we did everything we could to give these guys a chance. At this point, we feel like giving up but spent so much money already. Please help. Any advice on what we did wrong or right? Any advice on what to do going forward? I tested the water this morning with the one fish left. Our levels are: Nitrate 20, Nitrite 0, GH 150, Chlorine 0, KH 40-80, PH 7.2-7.8, Ammonia 0. Thank you for reading this super duper long post but I wanted to share as much info as I could.