I played myself.... I was doing a 25 percent water change and put untreated tap water in my tank....... I noticed 1 minute after I put the water in and quickly put 5 ml of prime in my 29 gallon tank.DID THE BACTERIA DIE!! I HAVE 15 NERITES AND 6 MYSTERY SNAILS AND 1 DWARF GOURAMI.THOUGHTS, SUGGESTIONS HELP!!!