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If all you want is the look, an inch or two of sand will be fine. If you want it for the need of the fishes then you'll need anywhere from three to six inches of sand, depending on the fish. You only want to vacuum once a week, two tops, so as to not kill of much of the beneficial bacteria. You'll want to mix the sand up well once or twice a week (more than just vacuuming) if you are not going to have sand sifting critters. There are some types of vacuums, though more expensive, that allow you to vacuum the sand and substrate and then the vacuum itself filters it out so that the sand, substrate and most of the clean water go back in the tank. This saves you money in the long run by not having to replace as much of the sand and substrate as often. You might want to look into that, I know I will!
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