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The common pleco is ok with the guppies. That tank is just below what a pleco's minimum tank size (55g) is, but should be ok with maintenance to keep the water parameters right and the waste from the pleco under control.
I would keep the temp around 76F and consider 100% water change. First remove the fish to temporary containers or buckets that could be covered enough to prevent jumpers. Then clean the substrata and move it into buckets/containers that had enough treated water to cover the substrata.. you could also put the filter sponges and live plants (if any) in with the substrata. Then you could empty and clean the tank well, cleaning any decor and putting the decor with the substrata. Refill the tank to about 25%, put the substrata back in and decor, then fill it to near full. I'd put new filter floss in the filter and put the sponges back in after the filter was running. Lastly you could float the fish and acclimate them.
Guppies live about 2 years, give or take 1/2 year, I think.
If it were just the gups, I'd say about 25-30. With the Pleco in there, maybe half of that? See what others say before carving that guess-timate in stone.
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