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You'll for sure have a chance give the substrate a good cleaning this way.
alicem
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Personally I wouldn't clean the substrate as this is where the majority of your healthy bacteria lives.
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While a good amount of bacteria lives on the
surface of the substrate, it also lives on the walls of the tank,
on rocks and other decorations, on the fake and real plants, but I feel that the majority lives in the filter.
That is why we suggested (or I thought it was understood) to keep all of those components wet in external tubs during the move.
Completely changing out the substrate could cause a mini cycle, very true.
When I said to give it a good cleaning, I just meant a good gravel vac, not a total disinfecting...
Any time we clean or dismantle any part of the aquarium, there is a chance of bacteria bloom, especially in a newer tank setup.
Doing a good gravel vac shouldn't destroy the majority of the good bacteria.
It could stir up nitrates or anaerobic bacteria if not done on a routine basis, for sure.
I should have asked SCUGrad if he had been doing regular gravel vacs.
The advice would be to preform a good one, since the fish would be out of the tank and not be exposed to all that.
I just can't imagine moving a tank with fish in it.
Poor things sloshing around, possibly getting damaged and definately getting stressed, but that was me assuming...
Sorry if what I said was misleading...
Best of luck on your 40' tank move,
alicem