Hi noggin welcome to Fish Lore
Sorry about your gups and neons, they're more sensitive than they used to be.
Are you using the liquid or test strips?
Are they commom plecos? If so they need a much bigger tank, 55 and up and your clown loaches will also need a bigger tank as they can get large.
Just a guess, but even though your existing fish seem happy in the tank now, it's may not be possible to add anymore without the undue stress of an overstocked tank.
Don't know anything about water hardness and it's effects on neons and guppies, hopefully someone else will address that.
Good luck.