from the
nitrogen cycle page
Option 3:
Use 100% pure ammonia.
Using a dropper, add 5 drops of ammonia per 10 gallons of aquarium water. If you don't get an ammonia reading with your
test kit, add some more drops until you start to see an ammonia reading. Keep track of how many drops you've used so you can repeat this process daily. Continue to dose the tank with ammonia until you start to get nitrite readings with your test kit. Once you can detect nitrites you should only add 3 drops of ammonia per 10 gallons of aquarium water, or if you added more drops originally to get an ammonia reading cut the amount of drops used in half. Continue this process daily until you get nitrate readings with your test kit. Do a 30%
water change and your tank is ready.